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1植物名称毛冬青(Ilex pubescens Hook.et Arn.)。2材料类别成熟果实。3培养条件基本培养基为1/2MS。(1)种子无菌发芽培养基:1/2MS+6-BA 0.5 mg·L~(-1)(单位下同)+IBA0.2;(2)丛芽增殖培养基:1/2MS+6-BA 1.0+IBA 0.2;(3)生根培养基:1/2MS+6-BA 1.0+NAA 0.5。上述培养基中均加入20 g·L~(-1)蔗糖和5 g·L~(-1)琼脂,pH 5.8。培养温度为(25±2)℃,光照时间为8～10 h·d~(-1),光照
1 plant name Mao Dongqing (Ilex pubescens Hook.et Arn.). 2 material categories ripe fruit. 3 culture conditions Basic medium 1 / 2MS. (1) sterile seed germination medium: 1 / 2MS + 6-BA 0.5 mg · L -1 (unit same below) + IBA0.2; (2) Cong bud proliferation medium: 1 / 2MS + 6 -BA 1.0 + IBA 0.2; (3) rooting medium: 1/2 MS + 6-BA 1.0 + NAA 0.5. The above medium was added 20 g · L -1 sucrose and 5 g · L -1 agar, pH 5.8. The incubation temperature was (25 ± 2) ℃, the illumination time was 8 ~ 10 h · d ~ (-1), light