Expression and identification of recombinant soluble single—chain variable fragment of monoclonal an

• 作 者: HeFT NieYZ
• 机 构: ThirdMilMedUniv,FourthMilMedUniv
• 出 处: 世界胃肠病学杂志：英文版（电子版）
• 发表日期: 2002年2期
• 关键词: 单克隆抗体 MC3 重组可溶性单链可变片段 表达 识别 AnimalsAntibodies MonoclonalBase SequenceCarcinomaC

AIM: To generate soluble single chain variable fragments (ScFv) of monoclonal antibody MC3 recognizing colorectal and gastric carcinomas.METHODS: mRNA was isolated from the hybridoma cell lineproducing MC3 and the DNAs encoding variable domains ofheavy an