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采用分批培养试验和连续培养试验研究了基质性毒物(氨和亚硝酸)对厌氧氨氧化富集培养物的单独和联合抑制效应.试验结果表明,分批培养时,氨和亚硝酸对厌氧氨氧化富集培养物的半抑制浓度及95％可信限分别为1670.3(1518.3～ 1832.4)mg·L-1和565.3(239.0～916.3)mg·L-1,亚硝酸毒性大于氨;氨和亚硝酸对厌氧氨氧化富集培养物的联合抑制效应为独立作用,二者各自对厌氧氨氧化富集培养物产生毒害.在中性环境和高浓度基质条件,高浓度的游离氨和游离亚硝酸会抑制厌氧氨氧化富集培养物,防止游离态基质浓度过高是解决基质自抑制的重要手段.连续培养时,随着氨浓度的上升,反应器效能和联氨含量基本稳定;随着亚硝酸浓度的上升,反应器效能急剧下降,联氨快速累积.基质亚硝酸对厌氧氨氧化富集培养物的抑制源于联氨脱氢步骤受阻,细胞能量代谢不畅,所积累的中间产物联氨作为抑制物可能会进一步加剧抑制效应.“,”Batch culture tests and continuous culture tests were used to investigate the independent and joint inhibition effects of the substrate-toxicants ammonia and nitrite acid on anaerobic ammonia oxidation (Anammox) enrichment culture.The batch test results showed that the half-inhibitory concentration and 95％ confidence interval of ammonia and nitrite acid was 1670.3 (1518.3～ 1832.4)mg-L-1 and 565.3(239.0 ～ 916.3)mg· L-1,respectively.Nitrite acid is more toxic than ammonia.The joint inhibition effect of ammonia and nitrite acid was independent,which meant they did not disturb each other when inhibition happened.With the high concentrations of substrates in neutral medium,high concentrations of free ammonia and free nitrite acid were responsible for inhibition of anammox enrichment culture,indicating that reduction of substrates in free state was one of the most effective ways to avoid substrate self-inhibitions.The continuous test showed that the performances of anammox reactors and the concentrations of hydrazine kept stable under the high concentrations of ammonia.However,the performances of anammox reactors sharply declined and the concentration of hydrazine accumulated quickly.Under the condition that anammox enrichment culture was inhibited by nitrite acid,the hydrazine dehydrogenase might be inhibited and the energy metabolism of anammox enrichment culture was disturbed.Moreover,the aggravation of inhibition effect might happen because high concentration of hydrazine is also an inhibitor.