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锰与铁、铜均为化学性质相似的过渡金属元素,但关于锰与自由基介导的脂质过氧化作用(LPO)的关系研究报道较少,且有争论.微粒体脂质过氧化模型已被广泛应用于研究自由基反应及筛选抗氧化剂,本文通过观察加锰前后三种LPO模型中终产物丙二醛(MDA)浓度的变化,初步探讨了锰的抗氧化作用.1 材料与方法 (1)大鼠肝微粒体提取:选用本校生产的Wistar雄性大鼠,体重180±10g,钙沉淀法分离微粒体,实验前制成15～20mg蛋白/ml的悬液.(2)微粒
Manganese and iron, copper are chemically similar transition metal elements, but there are few reports on the relationship between manganese and free radical-mediated lipid peroxidation (LPO), and there is controversy.Microsomal lipid peroxidation model Has been widely used in the study of free radical reaction and screening of antioxidants.This paper observed the changes of malondialdehyde (MDA) concentration in end products of three LPO models before and after manganese addition, and discussed the antioxidative effect of manganese.Materials and Methods (1) Extraction of rat liver microsome: Wistar male rats of our school were selected and weighed 180 ± 10g. The microsomes were separated by calcium precipitation, and 15 ~ 20mg protein / ml suspension was prepared before experiment. (2)