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A large cloud of 40Ca+ is successfully trapped and manipulated in a linear ion trap.The axial length of the ion cloud is measured under a series of end-cap voltages.We propose a method of measuring the axial secular motion temperature of the ion cloud by analyzing its image on an electron-multiplying CCD.The method is based on the Boltzmann equation that the axial density distribution of ions at secular motion temperature T satisfies.The axial secular motion temperature of the ion cloud is also obtained by measuring the Doppler broadened line width.For the same trapping parameters,the axial secular motion temperature by analyzing the image of ion cloud is 840K and by fitting the experimental resonance line profile is 700K.