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本文对南阳市市售肉类、乳及乳制品、蔬菜、水产品等食品和人畜粪便、污水及土壤等环境样品共624份进行了单核细胞增生李斯特氏菌的分离鉴定,结果为:LB法阳性18份,检出率5.77％,以粪便检出率最高15.0％,其次分别为生羊肉、冷饮、生猪肉、熟肉,分别为12.5％、10.0％、8.0％、10.0％。市售蔬菜(黄瓜、香菜等)和土壤、鱼、虾均未检出单核细胞增生李斯特氏菌。依据此结果进行单核细胞增生李斯特氏菌生态分布的分析,对今后防止单核增生李斯特氏菌病和食物中毒的发生有积极的指导意义。LB和EB两种检验方法的同时操作,结果表明两种检验方法检出结果基本吻合,LB法检出率明显高于EB法,且便于操作,可作为今后大面积开展普查筛选时首选的分离方法。
In this paper, a total of 624 samples of meat, dairy and dairy products, vegetables, aquatic products and other environmental samples were collected and tested for Listeria monocytogenes. The results were as follows: 18 positive samples were detected by LB method, and the detection rate was 5.77%. The detection rate of faeces was 15.0%, followed by raw mutton, cold drink, raw pork and cooked meat respectively 12.5%, 10.0%, 8.0% and 10.0% respectively. Commercial vegetables (cucumber, coriander, etc.) and soil, fish, shrimp are not detected Listeria monocytogenes. Based on the results of Listeria monocytogenes ecological distribution of the analysis of future prevention of Listeriosis monocytosis and food poisoning have a positive guiding significance. LB and EB. The results showed that the detection results of the two methods were basically consistent, the detection rate of LB method was significantly higher than that of EB method, and it was easy to operate and could be used as the first choice for large-scale screening screening in the future method.