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目的:观察藤黄各制剂单次大剂量ig给药后大鼠消化系统病变情况,并比较藤黄炮制前后对大鼠肠上皮细胞株IEC-6毒性差异,探讨藤黄炮制减毒机制。方法:单次ig给予藤黄生品(200 mg.kg-1)、清水制藤黄(200 mg.kg-1)和藤黄酸(50 mg.kg-1),观察给药后大鼠的活动情况,于24 h后处死并采集大鼠心、肝、肾、食管、胃、十二指肠、空肠、回肠和结肠等主要脏器,观察大鼠口服给药后消化系统病变情况。以大鼠肠上皮细胞株IEC-6为研究对象,采用MTT法检测藤黄炮制前后对IEC-6细胞增殖的影响,采用倒置显微镜观察细胞形态。结果:病理切片结果显示,给药后大鼠的肝脏、肾脏、心脏、胃和食道均未见明显的毒性反应;但十二指肠、空肠有轻度至重度的毒性作用,表现为肠绒毛水肿。3种藤黄制剂均会引起给药大鼠肠道的毒性作用,生品藤黄所致的十二指肠和空肠水肿程度最严重,其次是清水制藤黄和藤黄酸。细胞的毒性结果显示,与空白组相比,藤黄生品及炮制品均可明显降低IEC-6细胞的活性(P<0.01)与形态,且呈一定的剂量相关性;与藤黄生品组相比,藤黄3种炮制品组对IEC-6细胞的增殖抑制作用(P<0.01)和形态变差的趋势均显著性降低。结论:藤黄单次大剂量ig给药后大鼠的病理变化及IEC-6细胞的毒性作用结果表明,藤黄经炮制后毒性显著降低。
OBJECTIVE: To observe the pathological changes of digestive system in rat after single high-dose ig administration of Garcinia cambogia and to compare the toxicity of enteral epithelium cell line IEC-6 before and after treatment with Garcinia cambogia. Methods: The rats were treated with 200 mg.kg-1 Garcinia cambogia (200 mg.kg-1), 200 mg.kg-1 water and 50 mg.kg-1 Gambogic. After 24 hours, the rats were sacrificed and the major organs such as heart, liver, kidney, esophagus, stomach, duodenum, jejunum, ileum and colon were collected and the pathological changes of digestive system were observed. The rat intestinal epithelial cell line IEC-6 was used as the research object. MTT assay was used to detect the proliferation of IEC-6 cells before and after treatment with Garcinia cambogia. The cell morphology was observed by inverted microscope. Results: The results of pathological examination showed that there was no obvious toxic reaction in the liver, kidney, heart, stomach and esophagus of the rats after administration; however, the duodenum and jejunum had mild to severe toxicity and showed villus Edema. All the three kinds of Garcinia cambogia could cause the toxic effect on the intestine of rats. The edema of duodenum and jejunum caused by Garcinia cambogia was the most serious, followed by the water-made Garcinia cambogia and Gambogic acid. The results of cell toxicity showed that compared with the blank group, both the ganoderma lucidum and the processed product could significantly reduce the IEC-6 cell activity (P <0.01) and the morphology, and showed a dose-dependent manner. Compared with the control group, The proliferation of IEC-6 cells (P <0.01) and the trend of morphological deterioration were significantly decreased in the three processed products of Garcinia cambogia. CONCLUSION: The pathological changes of rat and the toxic effects of IEC-6 after a single high-dose ig administration of Garcinia cambogia showed that the toxicity of Garcinia cambogia was significantly reduced after being treated.