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目的研究骨髓间充质干细胞(MSCs)对小鼠Lewis肺癌皮下移植瘤的影响。方法自C57BL/6小鼠骨髓分离MSCs,制备单细胞悬液并于体外传代培养,取第4～5代细胞用于实验。56只C57BL/6小鼠经Lewis肺癌细胞皮下接种建立小鼠肺癌皮下移植瘤模型,根据MSCs给予时间分为D0组(接种同时给予MSCs)和D10组(接种后第10天给予MSCs),其中D0组分为3个亚组(n=8):组1单纯接种肿瘤细胞,组2肿瘤细胞和MSCs共同接种,组3肿瘤细胞接种及尾静脉注射MSCs;D10组分为4个亚组(n=8):组4(肿瘤细胞接种及瘤体内注射MSCs)及其等量PBS对照组(组5),组6(肿瘤细胞接种及尾静脉注射MSCs)及其等量PBS对照组(组7)。观察各组移植肿瘤的生长情况,包括肿瘤形成时间及不同时间点的瘤体大小,并进行组间分析比较。结果与组1和组3比较,组2的肿瘤形成时间明显缩短(P<0.05);而各时间点三组瘤体大小比较,差异无统计学意义(P>0.05)。组4的瘤体显著大于其对照组(P<0.05);而组6与其对照组的瘤体大小比较,差异无统计学意义(P>0.05)。结论MSCs与Lewis细胞同时接种可加速小鼠肺癌皮下移植瘤形成,而成瘤后MSCs瘤体内注射具有促进移植瘤生长的作用。
Objective To study the effect of bone marrow mesenchymal stem cells (MSCs) on the subcutaneous xenografts of Lewis lung carcinoma in mice. Methods MSCs were isolated from bone marrow of C57BL / 6 mice, and single cell suspension was prepared and subcultured in vitro. The 4th to 5th passage cells were used for the experiment. Fifty-six C57BL / 6 mice were subcutaneously inoculated with Lewis lung cancer cells to establish a subcutaneous xenograft model of lung cancer in mice. The mice were divided into D0 group (given MSCs simultaneously) and D10 group (given MSCs on the 10th day after inoculation) Group D0 was divided into three subgroups (n = 8): group 1 was inoculated with tumor cells alone, group 2 tumor cells and MSCs co-inoculated, group 3 tumor cells were inoculated and tail vein injected with MSCs; group D10 was divided into 4 subgroups n = 8): group 4 (tumor cell inoculation and intratumoral injection of MSCs) and its equivalent PBS control group (group 5), group 6 (tumor cell inoculation and tail vein injection of MSCs) and its equivalent PBS control group 7). The growth of tumor in each group was observed, including tumor formation time and tumor size at different time points. Results Compared with group 1 and group 3, the time of tumor formation in group 2 was significantly shorter (P <0.05). There was no significant difference in tumor size between the three groups at all time points (P> 0.05). The tumor size in group 4 was significantly larger than that in control group (P <0.05). There was no significant difference in tumor size between group 6 and the control group (P> 0.05). Conclusions Simultaneous inoculation of MSCs with Lewis cells can accelerate the formation of subcutaneous xenograft tumors in mice and the in vivo injection of MSCs can promote the growth of transplanted tumors.