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目的探讨羟喜树碱(HCPT)联合顺铂热化疗对人肝癌细胞的毒性增效作用,观察其腹腔热灌注治疗肝癌癌腹腔积液的疗效。方法流式细胞术检测温热条件下HCPT联合顺铂处理后肝癌细胞的凋亡。观察48例晚期肝癌腹腔积液患者热灌注化疗的疗效及不良反应。检测治疗前后血清AFP水平变化。结果 HCPT联合顺铂化疗显著增加对肝癌细胞的细胞凋亡率。热灌注HCPT联合顺铂治疗肝癌腹腔积液的有效率为60%(18/30),显著高于顺铂单药灌注组38.9%,不良反应轻微。血清AFP治疗后显著下降,且与疗效相关。结论 HCPT联合顺铂热化疗能显著诱导肝癌细胞凋亡,热灌注HCPT联合顺铂是晚期肝癌腹腔积液安全有效的治疗方法。
Objective To investigate the toxic effect of HCPT combined with cisplatin on human hepatocellular carcinoma cells and to observe the therapeutic effect of intraperitoneal hyperthermic perfusion on the treatment of hepatocellular carcinoma ascites. Methods Flow cytometry was used to detect the apoptosis of hepatocellular carcinoma cells treated with HCPT and cisplatin under warm conditions. To observe the curative effect and adverse reactions of 48 patients with advanced hepatocellular carcinoma due to intraperitoneal perfusion chemotherapy. Serum AFP levels were measured before and after treatment. Results HCPT combined with cisplatin chemotherapy significantly increased the rate of apoptosis of hepatocellular carcinoma cells. Hyperthermic HCPT combined with cisplatin in the treatment of liver cancer, ascites effusion efficiency was 60% (18/30), significantly higher than the cisplatin monotherapy group 38.9%, minor adverse reactions. Serum AFP significantly decreased after treatment, and with the effect. Conclusion HCPT plus cisplatin can significantly induce apoptosis of hepatocellular carcinoma cells. HCPT combined with cisplatin is a safe and effective treatment for patients with advanced hepatocellular carcinoma.