论文部分内容阅读
目的同时测定鱼腥草注射液中萜品烯-4-醇、α-萜品烯醇和甲基正壬酮挥发性成分的含量。方法采用GC法,色谱柱为SE-54毛细管柱(30 mm×0.32 mm,0.25μm),升温程序为:以2℃.min-1从80℃升至120℃,再以10℃.min-1升至200℃,保持5 min。以正十六烷为内标,选用C18固相萃取小柱对样品进行纯化浓集。结果萜品烯-4-醇0.03~0.30 mg.ml-1线性关系良好(r=0.9996),平均回收率为98.5%(RSD=1.0%);α-萜品烯醇0.01~0.10 mg.ml-1的线性关系良好(r=0.9998),平均回收率为98.8%(RSD=0.2%);甲基正壬酮0.01~0.10 mg.ml-1的线性关系良好(r=0.9999),平均回收率为99.9%(RSD=1.8%)。结论所建方法准确、简便、快速,可用于测定鱼腥草注射液中的3种挥发性成分。
Objective To determine the contents of volatile components of terpinen-4-ol, α-terpineol and methyl-n-nonyl ketone in Houttuynia cordata Thunb. Methods GC method was used. The chromatographic column was SE-54 capillary column (30 mm×0.32 mm, 0.25 μm). The temperature increase program was: 2°C.min-1 from 80°C to 120°C, then 10°C.min- 1 liter to 200°C and hold for 5 minutes. With hexadecane as the internal standard, the sample was purified and concentrated using the C18 solid phase extraction cartridge. Results The linear relationship of terpinen-4-ol 0.03-0.30 mg.ml-1 was linear (r=0.9996). The average recovery was 98.5% (RSD=1.0%); α-terpineol was 0.01-0.10 mg.ml The linear relationship of -1 was good (r=0.9998), the average recovery was 98.8% (RSD=0.2%); the linear relationship of methyl n-nonanone 0.01 to 0.10 mg.ml-1 was good (r=0.9999), the average recovery The rate was 99.9% (RSD = 1.8%). Conclusion The established method is accurate, simple and rapid. It can be used to determine the three volatile components in Houttuynia injection.