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本文旨在建立小鼠后肢微创H反射模型,为临床脊髓损伤等相关疾病研究提供一个简便易行的诱导H反射的实验方法。对浅麻醉昆明小鼠后肢进行微创手术:在下肢股骨大转子与尾根部连线中外1/3交点处切开皮肤,将一对细铜线分别穿经浅层肌肉并绑扎固定于坐骨神经附近组织,作为第一对刺激电极;在踝部以上跟腱内侧靠近胫神经处浅层肌肉穿入另一对电极,作为第二对刺激电极;将细铜线穿过跖骨近端表面皮肤并绑扎固定作为记录电极;参考电极置于后肢拇趾根部足垫。把相应电极连接到肌电图诱发电位仪,记录M波和H波,并利用在下肢两不同刺激点所诱导的M波和H波潜伏期的差值测量神经传导速度。此模型H反射引出率在坐骨神经和胫神经处分别为92.73%和81.82%,H波潜伏期约为7～10ms。测得运动神经传导速度(motor nerve conduction velocity,MNCV)和感觉神经传导速度(sensory nerve conduction velocity,SNCV)分别为(25.84±4.70)和(31.45±7.31)m/s。这种在小鼠后肢建立的微创H反射模型简便易行,对小鼠损伤轻微,所获得的波形干扰小,非常适用于对H反射潜伏期的研究以及需要小鼠较长时间保持良好生理状态的实验项目。此模型也可用于感觉和运动神经的传导速度检测。
This article aims to establish mouse hindlimb minimally invasive H reflex model for the study of clinical spinal cord injury and other related diseases to provide a simple and easy method of induction of H reflex experimental method. Minimally invasive surgery was performed on the hind limbs of the light anesthetized Kunming mice: the skin was cut at 1/3 of the suture between the greater trochanter and the tail root of lower extremity, and a pair of thin copper wires were respectively penetrated through the superficial muscles and ligated around the sciatic nerve Tissue as the first pair of stimulation electrodes; penetrate another pair of electrodes near the anterior to the tendon in the ankle near the tibial nerve to serve as a second pair of stimulation electrodes; pass a thin copper wire through the skin of the proximal metatarsal surface and ligature Fixed as a recording electrode; reference electrode placed in the hind foot toe base foot pad. The corresponding electrodes were connected to the electromyogram evoked potentiometer to record the M wave and the H wave, and the nerve conduction velocity was measured by using the difference between the M waves and the H waves induced by two different stimulation points of the lower limbs. The rate of H reflex in this model was 92.73% and 81.82% respectively in the sciatic nerve and tibial nerve, and the latent period of H wave was about 7 ~ 10ms. The motor nerve conduction velocity (MNCV) and sensory nerve conduction velocity (SNCV) were measured as (25.84 ± 4.70) and (31.45 ± 7.31) m / s, respectively. The minimally invasive H reflex model established in the mouse hindlimb is simple and easy to damage the mice, the waveform interference obtained is small, very suitable for the study of H reflex latency and the need for mice to maintain a good physiological state for a long time Experimental project. This model can also be used for sensory and motor nerve conduction velocity testing.