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以黄瓜品种“津优35”为试材,播种时采用接种和不接种丛枝菌根真菌(AMF)地表球囊霉(Glomus versiforme)以及幼苗期喷施不同浓度(0、0.50、0.75、1.00、1.25、1.50mmol·L~(-1))水杨酸(salicylic acid,SA),并进行低温(昼夜温度为12℃/5℃)胁迫的处理方法,研究了不同处理对黄瓜植株耐寒性的影响。结果表明:与不接种的相比,低温胁迫下相同SA浓度处理下接种地表球囊霉显著提高了黄瓜根系的活力、叶片叶绿素含量、可溶性糖含量和可溶性蛋白质含量;降低叶片丙二醛(MDA)含量和细胞膜透性。其中,以施加外源SA浓度1.00mmol·L~(-1)配合接种地表球囊霉提高黄瓜抗低温的效果最佳。可知SA与AMF处理具有改善冬季保护地黄瓜生长的潜力。
Cucumber variety “Jinyou 35” was used as test material. Inoculation and non-inoculation with AMF Glomus versiforme and seedling spraying with different concentrations (0, 50.50, 0.75 , 1.00, 1.25, 1.50 mmol·L -1) salicylic acid (SA) were selected and the treatments of low temperature (12 ℃ / 5 ℃) Impact of cold resistance. The results showed that inoculation of G. gossypii at the same SA concentration under low temperature stress significantly increased root activity, leaf chlorophyll content, soluble sugar content and soluble protein content as compared with non-inoculated ones, and decreased the content of malondialdehyde (MDA) ) Content and cell membrane permeability. Among them, the effect of applying low concentration of SA (1.00 mmol·L -1) to inoculation with S. gossypii to increase cucumber’s anti-hypothermia was the best. It can be seen that SA and AMF treatments have the potential to improve cucumber growth in winter.