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目的分析医源性表皮葡萄球菌生物膜基因型,探讨医源性表皮葡萄球菌ica操纵子与聚氯乙烯(PVC)材料表面细菌生物膜形成的关系。方法应用PCR法、DNA凝胶成像及基因测序技术检测医源性表皮葡萄球菌的生物膜形成相关基因,采用半定量测定方法测定PVC材料表面表皮葡萄球菌的细菌生物膜形成能力。结果医源性表皮葡萄球菌16S rRNA阳性率为100.0%;icaADB、atlE、fbe阳性基因型菌株占57.1%;icaADB阴性,atlE、fbe阳性基因型菌株占37.5%;ica操纵子阳性表皮葡萄球菌在PVC材料表面的细菌生物膜形成能力较ica操纵子阴性明显增强,差异有统计学意义(P<0.01)。结论医源性表皮葡萄球菌可以分为ica操纵子阴性和ica操纵子阳性两类细菌,ica操纵子可以增加PVC材料表面细菌生物膜的形成能力,这可能是区分共生性和侵袭性表皮葡萄球菌的遗传基础。
Objective To analyze the genotypes of iatrogenic Staphylococcus epidermidis biofilm and to explore the relationship between ica operon of iatrogenic Staphylococcus epidermidis and bacterial biofilm formation on the surface of polyvinyl chloride (PVC) material. Methods The biofilm-forming genes of staphylococcus epidermidis were detected by PCR, DNA gel imaging and gene sequencing. The biofilm formation ability of Staphylococcus epidermidis on PVC surface was determined by semi-quantitative method. Results The positive rate of 16S rRNA of iatrogenic Staphylococcus epidermidis was 100.0%. The positive strains of icaADB, atlE and fbe accounted for 57.1%, the negative ones were icaADB, the strains of atlE and fbe positive accounted for 37.5%. The ica operon positive Staphylococcus epidermidis The bacterial biofilm formation ability on the surface of PVC material was significantly higher than that of ica operon, with significant difference (P <0.01). Conclusion The iatrogenic Staphylococcus epidermidis can be divided into two types: ica operon and ica operon. The ica operon can increase the formation of bacterial biofilm on the surface of PVC material, which may be the difference between symbiotic and invasive Staphylococcus epidermidis The genetic basis.