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观察钙通道、钙调蛋白拮抗剂对人脐带静脉内皮细胞分泌内皮素-1（ET－1）的作用，结果表明钙通道拮抗剂异搏定（5．5×10－6及5．5×10-5mol／L）、硫氮办公酮（2．4×10-4mol／L）和钙调蛋白拮抗剂氯丙嗪（3．1×10-5及3．1×10-4mol/L）、小檗胺（1．6×10-5及1．6×10-4mol／L）均能明显抑制内皮细胞分泌ET－1，提示ET－1的分泌或释放受钙通道及细胞内钙调蛋白的调节。此外，硝酸甘油也明显抑制ET－1的分泌，提示一氧化氮有抑制ET－1分泌的作用。
To observe the effect of calcium channels and calmodulin antagonists on the secretion of endothelin-1 (ET-1) from human umbilical vein endothelial cells. The results showed that calcium channel antagonist verapamil (5.5 × 10-6 and 5.5 × 10-5mol / L), thiaminone (2.4 × 10-4mol / L) and calmodulin antagonist chlorpromazine (3.1 × 10-5 and 3.1 × 10-4mol / L) , And berbamine (1.6 × 10-5 and 1.6 × 10-4mol / L) significantly inhibited the secretion of ET-1 by endothelial cells, suggesting that the secretion or release of ET-1 is regulated by calcium channel and intracellular calcium Protein regulation. In addition, nitroglycerin also significantly inhibited the secretion of ET-1, suggesting that nitric oxide can inhibit the secretion of ET-1.