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目的研究板层蛋白在肾小球疾病时质或量的改变，探讨它们在肾脏中的生理功能和病理学意 义。方法本研究以板层蛋白α１，α２，β１，β２，γ１结构链为观察对象，用免疫组织化学和原位杂交技术研究它们在４８ 例肾小球疾病病理肾活检组织中的表达和分布是否发生质或量的改变。结果ＬＮα１，α２，β１和γ１的ｍＲＮＡ在伴系 膜增生的肾小球内表达有不同程度的增加，增生的系膜细胞是主要的细胞来源。在肾小球系膜增殖节段和硬化病 灶附近伴有ＬＮα１和γ１的蛋白增多和ＬＮα２，β１蛋白的异常表达，而ＬＮβ２链在相应节段的系膜区和ＧＢＭ表达出现 不同程度的下降。结论在系膜增生的肾小球疾病中，增生的系膜细胞是肾小球内板层蛋白量的积聚和异常合成 的重要细胞来源。在肾小球疾病中，板层蛋白的合成出现量和质的变化，这可能是肾小球疾病进展和恶化的物质 基础。
Objective To study the changes of the quality and quantity of lamellar proteins in glomerular diseases and to explore their physiological functions and pathological significance in the kidney. Methods In this study, the structural strands of lamina α1, α2, β1, β2 and γ1 were taken as the observation objects. Immunohistochemistry and in situ hybridization were used to investigate their expression and distribution in 48 cases of glomerular pathological renal biopsies A qualitative or quantitative change occurred. Results The mRNA expressions of LNα1, α2, β1 and γ1 were increased in glomeruli with mesangial hyperplasia. Mesangial cells were the main source of cells. The increased expression of LNα1 and γ1 protein and the abnormal expression of LNα2 and β1 protein in mesangial proliferative and sclerotic lesions of glomeruli, while the expression of LNβ2 decreased in some extent in mesangial and GBM of corresponding segments. Conclusions In mesangial proliferative glomerular diseases, mesangial cells proliferate and are important cellular sources for the accumulation and abnormal synthesis of glomerular platelet proteins. In glomerular diseases, the presence and quality of lamellar protein synthesis changes, which may be the material basis for the progression and worsening of glomerular disease.