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目的 探讨人乳铁蛋白对电离辐射后小鼠生存率以及对抗氧化的影响,研究人乳铁蛋白防治辐射损伤的作用及其机制.方法 小鼠随机分成三组,即正常对照组、照射对照组和乳铁蛋白防护组.乳铁蛋白防护组的小鼠给予乳铁蛋白溶液3ml灌胃,(1.0 g/1 hLF,灌胃前30 min生理盐水配制,室温放置),正常对照组和照射对照组给与等量生理盐水灌胃,每组每天一次,连续7d或30 d,末次给药2h后照射对照组和乳铁蛋白防护组的小鼠接受一次性X射线2 Gy或6.8 Gy全身均匀照射.7 d/2 Gy研究比较人乳铁蛋白对血清SOD活性、MDA含量的影响,以及肝脏组织形态学的变化,30 d/6.8 Gy研究观察小鼠生存质量、生存率.结果 给予乳铁蛋白后可显著抑制受照射小鼠血清SOD活性的降低以及MDA含量的升高,同时也可减轻肝脏细胞的凋亡,并可提高小鼠辐射后30d的生存率.结论 人乳铁蛋白可提高受到辐射后小鼠机体的抗氧化能力,提高小鼠的生存率,提示其具有一定辐射防护作用.“,”Objective To study its influence on the 30th day suivival rate,the antioxidant in mice and to study the preventive effects of hLF on radiation damage as well as its possible mechanism.Methods The mice were randomly divided into three groups:normal control group 、radiated control group and human lactoferrin protection group.The normal and radiated control groups were given isotonic Na chloride,once a day in seven days pre-irradiation.But protection group was given hLF,Mice in radiated control and protection group were exposed to X-ray after the last adminstration.All indexes were measured in the 24 h after radiation.Indexes include the 30-day survival rate of mice,the content of MDA,the SOD activity.The hepatic histopathologic changes were observed by microscopy.Results hLF could raise the 30-days survival rate of mice,and minimize the increasing degree of MDA and the decreasing degree of the activities of SOD.About protection group,liver damage reduced.Conclusion hLF can raise survival rate of radiated mice,enhance the their antioxidant ability.So we can conclude that the hLF may have some preventive effects on mice exposed to radiation.