Direct cloning and transplanting of large DNA fragments from Escherichia coli chromosome

来源 :中国科学：生命科学英文版 | 被引量 : 0次 | 上传用户：a7281423123

【摘要】

：

We applied a resistance split-fusion strategy to increase the in vivo direct cloning efficiency mediated by Red recombination.The cat cassette was divided into

【作者】

：

Ying Zhu Yan Yang Pingping Den

【机构】

：

InstituteofBiotechnology,InstituteofLifeScienceandtechnology,InstituteofHealthSciences,InstituteofMi

【出处】

：

中国科学：生命科学英文版

【发表日期】

：

2016年10期

【关键词】

：

DNA片段 Z染色体直接克隆大肠杆菌微生物天然产物移植同源序列生物合成途径 Red homologous recombination resist

【基金项目】

：

This work was supported by the National Natural Science Foundation of China （81373286）, and National Basic Research Program of China （2011CBAO0800）.

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We applied a resistance split-fusion strategy to increase the in vivo direct cloning efficiency mediated by Red recombination.The cat cassette was divided into two parts: cma(which has a homologous sequence with cmb) and cmb, each of which has no resistan

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