用酯酶同工酶检测香菇杂种

• 作 者: 张厚铿 孙慧明 舒娅玲
• 机 构: 江西省林科所林产利用室,江西省林科所林产利用室,江西省林科所林产利用室 南昌,南昌,南昌
• 出 处: 中国食用菌
• 发表日期: 1986年02期
• 关键词: 酯酶同工酶 异核体 遗传特征 生物科学 生化指标 文中 分离胶 杂交后代 秋栽 时间测定

酯酶同工酶在细胞质园球体中存在较多,其酶谱具有较稳定的遗传特征,故人们常用为检测遗传行为的一种生化指标。在近代生物科学中,特别是农业上多用同工酶来鉴别杂种及作为分类的依据。T.ToyomasuA.Zenyozi 在《同工酶电泳法鉴别香菇菌株》一文中指出:各菌株间的酯酶同工酶差异较大,可用来鉴别香菇菌株。试验采用聚丙烯酰胺凝胶电泳测定香菇杂交异核体63F 及其亲本的酯酶同工酶酶谱,以期检测香菇真杂种及杂种优势。

Esterase isoenzymes in the cytoplasm of the sphere there are many, and its zymogram has a more stable genetic characteristics, it is commonly used to detect genetic behavior of a biochemical indicators. In modern biological sciences, especially in agriculture, isozymes are used to identify hybrids and serve as a basis for classification. T.ToyomasuA.Zenyozi in the “isozyme electrophoresis identification of mushroom strains,” a paper pointed out: Esterase isolates between the different strains can be used to identify Lentinula edodes. The polyacrylamide gel electrophoresis was used to determine the esterase isozymes of the hybrid heterokaryon 63F and its parents to detect the true hybrids and heterosis of the mushrooms.