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目的:体外考察鼻咽清颗粒对鼻咽癌CNE-2细胞的抑制和放射增敏作用。方法:以体外培养的鼻咽癌CNE-2细胞为研究对象,MTT法考察鼻咽清颗粒对CNE-2细胞的增殖抑制作用,克隆形成实验检测鼻咽清颗粒对CNE-2细胞的放射增敏效应,流式细胞仪检测鼻咽清颗粒对CNE-2细胞周期和细胞凋亡的影响。结果:鼻咽淸颗粒浓缩液能抑制CNE-2细胞的增殖,其抑制作用呈时间-剂量依赖性;24,48和72 h的IC_50分别70.79,60.13,51.63 mg·ml-1(按主药材质量计);集落形成实验表明鼻咽清颗粒的浓缩液联合放射能明显降低CNE-2细胞集落形成,随着主药浓度的增加,CNE-2细胞集落形成减少,阴性对照组、单纯放射组、10 mg·ml-1和20 mg·ml-1(按主药材的重量计)鼻咽清浓缩液的集落形成数差异具有统计学意义(P<0.05);随着培养基中鼻咽清主药含量的增加,G2/M期所占的百分比提高,凋亡细胞所占的比例也相应增加,与对照组相比差异具有统计学意义(P<0.05)。结论:鼻咽清颗粒可抑制鼻咽癌CNE-2细胞增殖,将CNE-2细胞阻滞于G2/M期,诱导鼻咽癌细胞凋亡,具有放疗增敏作用。
OBJECTIVE: To investigate the inhibitory and radiosensitizing effects of nasopharyngeal carcinoma granule on CNE-2 cells in vitro. Methods: The nasopharyngeal carcinoma CNE-2 cells were cultured in vitro. MTT assay was used to detect the inhibitory effect of nasopharyngeal carcinoma granule on the proliferation of CNE-2 cells. The clonogenic assay was used to detect the proliferation of CNE-2 cells Sensitization, flow cytometry nasopharyngeal particles on cell cycle and apoptosis of CNE-2. Results: The nasopharyngeal pellet concentrate could inhibit the proliferation of CNE-2 cells in a time-and dose-dependent manner. The IC50 values of CNE-2 cells were 70.79, 60.13 and 51.63 mg · ml-1 at 24, 48 and 72 h The results of colony formation assay showed that CNE-2 cells colony formation was significantly reduced by concentrated nasopharyngeal granules and radioactivity, and the colony formation of CNE-2 cells decreased with the increase of the main drug concentration. The negative control group, radiotherapy alone group , 10 mg · ml-1 and 20 mg · ml-1 (based on the weight of the main drug) nasopharyngeal clear concentrate colony formation difference was statistically significant (P <0.05); with the medium of nasopharyngeal clear The main drug content increased, G2 / M phase percentage increased, the proportion of apoptotic cells also increased accordingly, compared with the control group, the difference was statistically significant (P <0.05). CONCLUSION: Nasopharyngeal Granule can inhibit the proliferation of CNE-2 cells, arrest CNE-2 cells in G2 / M phase, induce the apoptosis of nasopharyngeal carcinoma cells and radiosensitize the cells.