食品中双歧杆菌计数LGC能力验证结果与分析

• 作 者: 王似锦 刘文杰 张光华 牛振东 井良义 江志杰 高春
• 机 构: 北京市药品检验所(北京市保健食品化妆品检验中心),
• 出 处: 首都食品与医药
• 发表日期: 2015年16期
• 关键词: 能力验证 LGC 检验能力 锂盐 微生物室 DNA 莫匹罗星 通用引物 厌氧 作业指导书

目的通过参加LGC国际能力验证,提高食品中双歧杆菌计数的检验能力。方法法分别用添加莫匹罗星锂盐的TOS培养基和MRS培养基,37℃培养进行双歧杆菌计数,并用16Sr DNA测序的方法对得到的细菌进行鉴定。结论双歧杆菌计数的结果为2700cfu/g,鉴定为短双歧杆菌。通过结果的反馈,此次能力验证取得了满意的结果(Z=0.37)。

Objective To improve the testing ability of bifidobacteria count in food by participating in LGC International Competence Verification. Methods Methods: The Bifidobacterium was counted by using TOS medium and MRS medium containing mupirocin lithium salt, cultured at 37 ℃, and the bacteria were identified by 16Sr DNA sequencing. Conclusion The result of Bifidobacterium counting was 2700cfu / g, which was identified as Bifidobacterium breve. Through the feedback of the results, this proficiency testing achieved satisfactory results (Z = 0.37).