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许多流行病学调查结果揭示,乙型肝炎病毒(HBV)与原发性肝癌(PHC)有病原学联系。无论是在人,还是在动物模型均已观察到 PHC 主要发生在病毒的慢性感染基础上。HBV DNA 整合入肝细胞DNA,常常伴随了两者基因的强烈重排现象,因此 HBV 能否引起肝细胞癌变的问题是当前研究 PHC 发生机理的着重点。早在1980年,Brechot 等运用 Southern印迹技术首次从人肝癌组织和 PLC/PRF/5细胞株提取的 DNA 中检测出整合型的
Many epidemiological investigations have revealed that HBV is associated with primary liver cancer (PHC). PHC has been observed to occur primarily on the basis of chronic viral infections, both in humans and in animal models. The integration of HBV DNA into hepatocyte DNA is often accompanied by a strong rearrangement of both genes. Therefore, whether HBV can cause hepatocellular carcinogenesis is the focus of current research on the mechanism of PHC. As early as 1980, Brechot et al. detected the integrated type of DNA extracted from human hepatocellular carcinoma and PLC/PRF/5 cell lines using Southern blot technology for the first time.