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以崇明水仙种球为试材,采用ELISA法(酶联免疫法),研究了内源激素含量的变化规律对分别贮藏于20℃恒温和室温条件下,贮藏期内种球花芽分化的影响,以期为今后崇明水仙的花期调控等研究提供参考依据。结果表明:贮藏期间种球内的赤霉素(GA)含量均上升直至第7周,20℃处理组的峰值高出室温组40.67%。生长素(IAA)含量在2种贮藏条件下的变化略有差异,室温组IAA含量在第3~6周均高于20℃处理组,而20℃处理组的花芽分化进程在这4周中明显快于室温组。室温组玉米素核苷(ZR)含量变化与GA趋势一致,但20℃处理组的变化略有差异,直至取样结束,仍处于上升态势。2组处理的脱落酸(ABA)含量基本均呈现出先降后升的变化趋势,都在第3周出现最低值,室温组最低值低于20℃处理组16.97%,随后20℃处理组上升明显较室温组快。由此可知,相对低温可以加速水仙种球的花芽分化。20℃恒温贮藏对水仙花芽分化有促进作用,有利于GA含量的积累,且较高水平的GA含量可以加速花芽分化。高水平的ZR与促进花芽分化进程有密切关系。内源IAA和ABA水平则与花芽分化呈负相关。
The effect of endogenous hormones content on the differentiation of flower buds of bulbs in storage period at 20 ℃ and room temperature was studied by using ELISA method (enzyme-linked immunosorbent assay) With a view to provide reference for the research on flowering regulation of Narcissus in Chongming in the future. The results showed that the content of gibberellin (GA) in the bulbs increased until 7th week, and the peak value at 20 ℃ was 40.67% higher than that in the room temperature group. The contents of IAA changed slightly under the two storage conditions. The content of IAA in room temperature was higher than that in 20 ℃ in the 3rd ~ 6th week, while the flower bud differentiation in 20 ℃ was obviously faster in these 4 weeks Group at room temperature. At room temperature, the change of zeatin riboside (ZR) content was consistent with that of GA, but there was a slight difference in the change of 20 ℃ treatment group, which was still in the rising trend until the end of sampling. The contents of ABA in the two treatments all showed the trend of decreasing first and then rising, all of them appeared the lowest value in the third week, the lowest value in the room temperature group was lower than that of the treatment group of 20 ℃, and then increased significantly at 20 ℃ Faster than room temperature group. It can be seen that the relatively low temperature can accelerate the flower bud differentiation Narcissus bulbs. Storage at 20 ℃ in constant temperature could promote flower bud differentiation, promote the accumulation of GA, and higher GA could accelerate flower bud differentiation. High levels of ZR are closely related to the promotion of flower bud differentiation. Endogenous IAA and ABA levels were negatively correlated with flower bud differentiation.