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萘作为土壤动物化学抑制剂已在土壤动物生态功能的研究中广泛使用,但其非目标效应使其应用仍存在很大的不确定性.为了了解在亚高山森林土壤应用萘抑制土壤动物群落是否存在非目标效应,以青藏高原东缘的川西亚高山森林土壤为研究对象,采用微缩试验研究了萘对土壤呼吸速率、养分含量和酶活性的短期影响.结果表明:萘处理显著抑制了培养0~10 d的土壤呼吸速率,随后(24~52 d)表现出明显的促进作用.萘处理显著影响了土壤铵态氮和硝态氮含量的动态变化,萘处理铵态氮和硝态氮含量分别以培养的3和17 d最高,对照则以培养的45 d和结束时的52 d最高.萘处理土壤可溶性碳含量在培养3 d急剧增加后迅速降低,对照则略有升高后降低,而萘处理和对照的可溶性氮含量均表现为先升高后降低.萘处理和对照的土壤酶活性均具相似的动态规律,两者的脲酶、硝酸还原酶和亚硝酸还原酶活性分别在培养45、38和10 d至最高.萘处理和采样时间的交互作用显著影响了土壤呼吸速率,以及土壤铵态氮、硝态氮和可溶性氮含量,但对可溶性碳含量、蔗糖酶、硝酸还原酶和亚硝酸还原酶活性的影响不显著.萘作为驱虫剂的非目标效应可能在短期内对川西亚高山森林土壤的氮循环过程产生强烈的影响.
Naphthalene, as a chemical inhibitor of soil animals, has been widely used in the study of ecological function of soil animals, but its non-target effect still leaves great uncertainty in its application.In order to understand whether naphthalene inhibits soil fauna in subalpine forest soils There is a non-target effect on the eastern Qinghai-Tibet Plateau in sub-alpine forest soil as the research object, the micro-scale experiment was conducted to study naphthalene on soil respiration rate, nutrient content and enzyme activity of the short-term effects of naphthalene treatment significantly inhibited the culture 0 ~ 10 d soil respiration rate, and then (24 ~ 52 d) showed a significant role in promoting.Naphthalene treatment significantly affected the dynamic changes of soil ammonium nitrogen and nitrate nitrogen content, naphthalene treatment of ammonium nitrogen and nitrate nitrogen content Respectively, which were the highest at 3 and 17 d of culture respectively, and the highest at 45 d and 52 d at the end of the control.The soluble carbon content in the soil treated with naphthalene decreased rapidly after 3 d of culture and then increased slightly and then decreased, While the contents of soluble nitrogen in both naphthalene and control were increased firstly and then decreased.The activities of soil enzymes in naphthalene control and control all had similar dynamic laws.The urease and nitrate reductase Nitrite reductase activity was highest at 45, 38, and 10 d, respectively.The interaction of naphthalene treatment and sampling time significantly affected soil respiration rate, as well as soil ammonium nitrogen, nitrate nitrogen and soluble nitrogen content, but soluble carbon The content of invertase, sucrase, nitrate reductase and nitrite reductase activity had no significant effect.Non-target effect of naphthalene as a repellent might have a strong effect on the nitrogen cycle of subalpine forest soils in the west of China.