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目的 探讨 T细胞疫苗 ( TCV)接种对实验性自身免疫性脑脊髓炎 ( EAE)免疫机制的调节作用。方法 取正常及 EAE大鼠腹股沟淋巴结细胞 ,经 MBP抗原诱导 ,制备 MBP特异的 TCV用于接种 ,以 HE染色观察髓鞘病变 ,MTT法检测细胞毒反应 ,FACS方法检测 T细胞亚群 ,ELISA方法检测血清中细胞因子含量。结果 接种 TCV后 ,CD8+细胞百分率上升 ,T细胞对脑细胞的杀伤率下降 ,血清中 IFN-γ与 TNF-α含量下降以及脑髓质炎症反应减弱 ,EAE发病率下降。结论 特异性 TCV接种可降低自身免疫反应性。 TCV通过淋巴细胞亚群的变化及细胞因子的调节 ,发挥对 EAE的免疫预防和治疗效应
Objective To investigate the regulatory effect of T cell vaccine (TCV) vaccination on the immune mechanism of experimental autoimmune encephalomyelitis (EAE). Methods Normal and EAE rat inguinal lymph node cells were induced by MBP antigen, MBP-specific TCV was prepared for inoculation, the myelin lesions were observed by HE staining, cytotoxicity was detected by MTT assay, T cell subsets were detected by FACS and ELISA method Serum cytokine levels were measured. Results The percentage of CD8 + cells increased, the killing rate of T cells to brain cells decreased, the levels of IFN-γ and TNF-α in serum decreased, the inflammatory response of brain mass decreased, and the incidence of EAE decreased. Conclusion Specific TCV vaccination can reduce the autoimmune reactivity. TCV through the changes in lymphocyte subsets and cytokine regulation play an immunoprophylactic and therapeutic effect on EAE