论文部分内容阅读
目的掌握2012~2013年引起平顶山市手足口病的病原体型别及分布特征,为防控工作提供科学依据。方法采集平顶山市临床诊断为手足口病病例的粪便或肛拭子样本,应用实时荧光定量RT-PCR方法进行肠道病毒及EV71、CA16特异性核酸检测。结果 2012~2013年合计检测658份疑似手足口病标本中,检出阳性标本510份,阳性率77.51% 。其中2012年手足口病病原以EV71型肠道病毒为主,阳性率为52.35% (200/382);2013年手足口病病原以CA16型肠道病毒为主,阳性率为32.25% (89/276)。4~6月为发病高峰期,以4岁以下婴幼儿最多91.79% (604/658),性别比为1.46∶1。结论 EV71和CA16为该地手足口病的主要病原体。
Objective To grasp the types and distribution of pathogens causing hand-foot-mouth disease in Pingdingshan City from 2012 to 2013, and to provide a scientific basis for prevention and control. Methods Samples of feces or anal swab clinically diagnosed as hand-foot-mouth disease were collected from Pingdingshan City. Real-time fluorescence quantitative RT-PCR was used to detect enterovirus and EV71, CA16 specific nucleic acids. Results From 2012 to 2013, a total of 658 samples of suspected HFMD were detected, and 510 positive samples were detected, the positive rate was 77.51%. Among them, EV71 was the most common enterovirus in HFMD in 2012, with a positive rate of 52.35% (200/382). In 2013, the pathogen of HFMD was CA16 and the positive rate was 32.25% (89 / 276). From April to June, it was the peak of onset, with a maximum of 91.79% (604/658) of infants under 4 years of age and a sex ratio of 1.46:1. Conclusion EV71 and CA16 are the main pathogens of HFMD in this area.