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暴露于空气中的钛表面容易受到碳氢化合物的污染,本研究的目的是探讨不同程度的碳氢化合物污染对钛片表面生物活性及对成骨细胞黏附和增殖的影响。将酸蚀处理的钛片分成4组,用常规的保存方法分别储存不同的时间。通过扫描电镜,表面轮廓仪,X射线光电子能谱和接触角测量等手段分析钛片表面理化性能的变化,然后在钛片表面进行细胞培养试验。结果表明,钛片的表面形态不会随着储存时间的改变而改变,但是,钛片表面的化学组成随着储存时间的增加而改变,碳含量从25.99%增加到48.16%。另外,碳氢化合物污染还改变了钛片表面润湿性,从最初的亲水性变成了疏水性。细胞培养结果表明,碳氢化合物的污染不利于成骨细胞的黏附与增殖。因此,改良钛表面的保存方法以保持其表面活性是以后研究的方向。
The surface of titanium exposed to the air is easily contaminated by hydrocarbons. The purpose of this study is to investigate the effects of different degrees of hydrocarbon contamination on the biological activity of titanium surface and the adhesion and proliferation of osteoblasts. The etched titanium plates were divided into 4 groups, which were stored for different times by conventional methods. The change of physical and chemical properties of the surface of the titanium plate was analyzed by scanning electron microscopy, surface profiler, X-ray photoelectron spectroscopy and contact angle measurement. Then the cell culture test was carried out on the surface of the titanium plate. The results show that the surface morphology of the titanium plate does not change with the storage time. However, the chemical composition of the titanium plate surface changes with the increase of storage time, and the carbon content increases from 25.99% to 48.16%. In addition, hydrocarbon contamination also changes the wettability of the titanium surface, from the initial hydrophilic to hydrophobic. Cell culture results show that the pollution of hydrocarbons is not conducive to the adhesion and proliferation of osteoblasts. Therefore, to improve the preservation of titanium surface in order to maintain its surface activity is the direction of future research.