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AIM: To examine the existence of Nitric oxide/ cGMP sensitive store-operated Ca2+ entry in mouse fibroblast NIH/3T3 cells and its influence on matrix metalloproteinase (MMP) production and adhesion ability of fi broblasts. METHODS: NIH/3T3 cells were cultured. Confocal laser scanning microscopy was used to examine the existence of thapsigargin-induced store-operated Ca2+ entry in fibroblasts. Gelatin zymography and semiquantitative reverse transcriptase-polymerase chain reaction (RT- PCR) were employed to detect the involvement of [Ca2+]i and NO/cGMP in MMP secretion. The involvement of NO/ cGMP-sensitive Ca2+ entry in adhesion was determined using matrigel-coated culture plates. RESULTS: 8-bromo-cGMP inhibited the thapsigargin- induced Ca2+ entry in 3T3 cells. The cGMP-induced inhibition was abolished by an inhibitor of protein kinase G, KT5823 (1μmol/L). A similar effect on the Ca2+ entry was observed in 3T3 cells in response to a NO donor, (±)-S-nitroso-N-acetylpenicillamine (SNAP). The inhibitory effect of SNAP on the thapsigargin-induced Ca2+ entry was also observed, indicating NO/cGMP- regulated Ca2+ entry in 3T3 cells. Results of gelatin zymography assay showed that addition of extracellular Ca2+ concentration induced MMP release and activation in a dose-dependent manner. RT-PCR also showed that cGMP and SNAP reduced the production of MMP mRNA in 3T3 cells. Experiments investigating adhesion potentials demonstrated that cGMP and SNAP could upgrade 3T3 cell attachment rate to the matrigel-coated culture plates.CONCLUSION: NO/cGMP sensitive store-operated Ca2+ entry occurs in fi broblasts, and attenuates their adhesion potentials through its in? uence on MMP secretion.
AIM: To examine the existence of Nitric oxide / cGMP sensitive store-operated Ca2 + entry in mouse fibroblast NIH / 3T3 cells and its influence on matrix metalloproteinase (MMP) production and adhesion ability of fi broblasts. METHODS: NIH / 3T3 cells were cultured. Confocal laser scanning microscopy was used to examine the existence of thapsigargin-induced store-operated Ca2 + entry in fibroblasts. Gelatin zymography and semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) were employed to detect the involvement of [Ca2 +] i and NO The involvement of NO / cGMP-sensitive Ca2 + entry in adhesion was determined using matrigel-coated culture plates. RESULTS: 8-bromo-cGMP inhibited the thapsigargin-induced Ca2 + entry in 3T3 cells. The cGMP-induced inhibition was abolished by an inhibitor of protein kinase G, KT5823 (1 μmol / L). A similar effect on the Ca 2+ entry was observed in 3T3 cells in response to a NO donor, (±) -S-nitroso-N-acetylpenicillamine ). The inhibitory effect of SNAP on the thapsigargin-induced Ca 2+ entry was also observed, indicating NO / cGMP-regulated Ca 2+ entry in 3T3 cells. Results of gelatin zymography assay that addition of extracellular Ca 2+ concentration induced MMP release and activation in a dose -dependent manner. RT-PCR also showed that cGMP and SNAP reduced the production of MMP mRNA in 3T3 cells. Experiments investigating adhesion potentials were that cGMP and SNAP could upgrade 3T3 cell attachment rate to the matrigel-coated culture plates. CONCLUSION: NO / cGMP sensitive store-operated Ca2 + entry occurs in fi broblasts, and attenuates their adhesion potentials through its in? uence on MMP secretion.