论文部分内容阅读
目的:观察海水浸泡对血管内皮细胞迁移和血管生成的影响,为探讨海水致伤机制、制定科学救治原则提供实验依据.方法:建立ECV304细胞划痕创伤模型,分别给予生理盐水和人工海水浸泡处理,划痕创伤愈合修复分析观察细胞迁移能力;建立人食管鳞癌EC0156裸鼠皮下移植瘤模型,分别给予生理盐水和人工海水干预,监测肿瘤体积并绘制肿瘤生长曲线,观察肿瘤边缘血管的生成,免疫组织化学染色检测肿瘤组织内微血管密度.结果:海水浸泡后,ECV304细胞失去正常形态,并随浸泡时间的延长,呈明显“脱水”状态.在划痕后17h,对照组划痕已基本愈合,而实验组仍可见清晰划痕,且以实验4h组划痕尤其明显.与对照组相比,实验组细胞划痕愈合明显延迟,细胞迁移能力降低;与对照组相比实验组肿瘤边缘新生血管较少,前者肿瘤边缘新生血管较丰富,两者有显著性差异(微血管密度值:1.2±0.44vs3.2±0.83,P<0.05).实验组肿瘤生长缓慢,肿瘤体积较小(P<0.05).结论:海水浸泡能够降低血管内皮细胞迁移能力并抑制血管生成.
OBJECTIVE: To observe the effect of seawater immersion on migration and angiogenesis of vascular endothelial cells, and provide experimental evidence for exploring the mechanism of saltwater injury and establishing scientific treatment principles.Methods: ECV304 cell scratch wound model was established and treated with saline and artificial seawater immersion Wound healing repair analysis to observe cell migration ability; establish human esophageal squamous carcinoma EC0156 subcutaneous xenograft model in nude mice, respectively, given saline and artificial sea water intervention, monitoring the tumor volume and draw the tumor growth curve to observe the tumor edge of the blood vessel formation, Immunohistochemical staining was used to detect the microvessel density in tumor tissue.Results: ECV304 cells lost their normal morphology after seawater immersion, and showed obvious “dehydration” state after immersion for a long time The wound healing in the experimental group was obviously delayed and the cell migration ability was decreased compared with the control group. Compared with the control group, the tumor in the experimental group The number of neovascular edge is less, the former is more abundant at the edge of the tumor, with significant difference (microvessel density value: 1.2 ± 0 .44vs3.2 ± 0.83, P <0.05) .Tumor growth in the experimental group was slow and the tumor size was small (P <0.05) .Conclusion: Seawater immersion can reduce the migration of vascular endothelial cells and inhibit angiogenesis.