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Objective. To study thymus-dependent T cell development and T cell repertoire in human s ever com-bined immunodeficiencypatients after HLA-identical or haploid entical T cell-depleted allogeneic bone marrow transplantation.Methods .Blood samples were obtained from15SCID patients before transplantation and a t varying intervals thereafter.Quantitative competitive PCR assay and immunosco pe analysis of the T cell receptorVarepertoire were performed.Results. Before and within the first100days after transplantation,patients’ periphera l blood mononuclear cellpresented an oligoclonal or polyclonal skewed T cell repertoire,low T cell re-ceptor excision circlesvalues and pred ominance of CD45RO + T cell.In contrast,the presence of high numbers of CD45RA + T cells in bone marrowcirculation reconstituted SCID patients(>10 0days post-transplantation)correlated with active T cell production by the th ymus as revealed by high TREC values,and a polyclonal T cell repertoire demonst rated by a Gaussian distribution of Va-specific peaks.Conclusions.Within one year after BMT ,a normal T cell repertoire develops in SCID patients as a resu lt of thymic output.The T cell receptor diversity is highly and positively corr elated in these patients with TREC levels.
Objective. To study thymus-dependent T cell development and T cell repertoire in human s continued com-bined immunodeficiency patients after HLA-identical or haploid ent T cell-depleted allogeneic bone marrow transplantation. Methods. Blood samples were obtained from 15 SCID patients before transplantation and at varying intervals thereafter. Quantitative competitive PCR assay and immunosco pe analysis of the T cell receptor Varieties were performed. Results. Before and within the first 100 days after transplantation, patients’ periphera l blood mononuclear cell presented an oligoclonal or polyclonal skewed T cell repertoire, low T cell re -ceptor excision circlesvalues and predominance of CD45RO + T cell. Contrast, the presence of high numbers of CD45RA + T cells in bone marrow reconstituted SCID patients (> 10 0 days post-transplantation) correlated with active T cell production by the thymus as revealed by high TREC values, and a polyclonal T cell repertoire demonstrated by a Gaussi an distribution of Va-specific peaks.Conclusions. Whith one year after BMT, a normal T cell repertoire develops in SCID patients as a resu lt of thymic output. T cell receptor diversity is highly and positively corrlated in these patients with TREC levels .