Which form of collagen is suitable for nerve cell culture?

• 作 者: Mohsen Fathi Najafi Saber Zahri Fatemeh Vahedi Leila Esmaililian Toosi Nazila Ariaee
• 机 构: Razi Vaccine and Serum Research Institute,Departamnt of Biology,University of Mohaghegh Ardebili,Inf
• 出 处: Neural Regeneration Research
• 发表日期: 2013年23期
• 关键词: collagen hydrolyzed inverted attachment cultured Collagen trypsin colony bromide

In this study, we investigated the effects of hydrolyzed and non-hydrolyzed collagen and two-dimensional and three-dimensional collagen matrices on cell survival, attachment and neurite outgrowth of primary cultured nerve cells using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay and inverted microscopy. Hydrolyzed collagen facilitated nerve cell survival and neurite outgrowth, but it had no obvious influences on cell attachment. In contrast, non-hydrolyzed two-dimensional collagen matrix had no obvious effects on neurite outgrowth. These findings suggest that hydrolyzed collagen is an ideal nerve cell culture media.

In this study, we investigated the effects of hydrolyzed and non-hydrolyzed collagen and two-dimensional and three-dimensional collagen matrices on cell survival, attachment and neurite outgrowth of primary cultured nerve cells using 3- [4,5-dimethylthiazol-2- Hydrolyzed collagen facilitated nerve cell survival and neurite outgrowth, but it had no obvious influences on cell attachment. In contrast, non-hydrolyzed two-dimensional collagen matrix had no obvious effects on neurite outgrowth. These findings suggest that hydrolyzed collagen is an ideal nerve cell culture media.