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目的:建立河南不同产地茜草药材的HPLC指纹图谱分析方法,为茜草药材的质量评价提供参考依据。方法:采用高效液相色谱法,Phenomenex ODS C18柱(4.60 mm×250 mm,4μm),甲醇-0.2%磷酸水梯度洗脱,检测波长275 nm,流速0.8 mL·min-1,柱温25℃。结果:以大叶茜草素为参照峰,确定11个共有峰,测定了14批茜草药材HPLC指纹图谱与对照图谱的相似度。结论:所建立的方法可用于茜草药材指纹图谱测定,并可对其今后规范药用资源及质量评价提供科学依据。
Objective: To establish HPLC fingerprinting method of Rubia cordifolia from different areas of Henan, and provide reference for the quality evaluation of Rubia cordifolia. Methods: High performance liquid chromatography (HPLC) was performed on a Phenomenex ODS C18 column (4.60 mm × 250 mm, 4 μm) with a gradient of methanol-0.2% phosphoric acid. The detection wavelength was 275 nm and the flow rate was 0.8 mL · min- . RESULTS: Eleven common peaks were identified with the reference peak of magnolia extract, and the similarity between HPLC fingerprints and control maps of 14 batches of Rubia cordifolia was determined. Conclusion: The established method can be used to determine the fingerprint of Rubia cordifolia and provide a scientific basis for its future specification of medicinal resources and quality evaluation.