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本实验是用胰蛋白酶分别溶解,经不同抗卵透明带血清处理的猪、兔、大鼠和鲤鱼的卵,观察各组卵透明带被溶解的情况和速度。根据各组卵透明带溶解速度即抗酶溶解能力的差异,来判断在透明带上是否形成了抗原-抗体复合物,从而证实卵透明带上的抗原与抗体是否发生了特异性免疫反应,以及了解几种不同动物种间免疫交叉反应程度。
In this experiment, eggs of pigs, rabbits, rats and carps which were dissolved by trypsin and treated with different anti-zona pellucida serotypes were observed. The situation and speed of zona pellucida of each group were observed. According to the differences of zona pellucida dissolution rate, that is, the ability of anti-enzyme dissolution, the antigen-antibody complex was formed on the zona pellucida so as to confirm whether the antigen and the antibody on the zona pellucida had a specific immune response, and Understand the immune cross-reactivity among several different species of animals.