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妊娠12~16周人工流产胎儿大脑神经细胞用1640培养液和加入0.24mmol/L牛磺酸的同种培养液进行原代单层培养,用免疫组织化学Streptavidinperoxidase(SP)法鉴定神经细胞,用 ̄3H-TdR掺入和图像分析等方法对脑神经细胞的生长发育、增殖分化、形态学变化及生存时间进行了研究。结果显示:牛磺酸组神经细胞的胞体面积、周长、突起长度、神经特异性烯醇化酶阳性率及 ̄3H-TdR掺入量均显著高于对照组(P<0.01),牛磺酸组细胞体外生存时间(96±4d)比对照组(64±3.3d)显著延长(P<0.01)。说明牛磺酸有促进人胚脑神经细胞的生长发育、增殖分化和延缓衰老的作用。
Twelve to sixteen weeks of gestation, aborted fetal brain neurons were cultured in primary monolayer with 1640 culture medium and 0.24 mmol / L taurine plus the same culture medium, and neurons were identified by immunohistochemistry Streptavidin peroxidase (SP) Using 3H-TdR incorporation and image analysis methods to study the growth, proliferation and differentiation, morphological changes and survival time of brain nerve cells. The results showed that the cell body area, perimeter, length of protuberance, neuron specific enolase positive rate and the amount of 3H-TdR incorporation in the taurine group were significantly higher than those in the control group (P <0.01) The survival time of sulfonate group in vitro (96 ± 4 days) was significantly longer than that of control group (64 ± 3.3 days) (P <0.01). Taurine can promote human embryonic brain cells in the growth and development, proliferation and differentiation and anti-aging effect.