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目的:测定双黄连注射液中绿原酸及黄芩苷含量。方法:采用 HPLC 法,色谱柱:C_(18)柱(5μm,250mm×4.6mm),流动相:甲醇-0.2 mol·L~(-1)磷酸二氢钠(磷酸调 pH=2.7)(44:56),流速:1.0 mL·min~(-1),检测波长:320nm。结果:绿原酸和黄芩苷进样量分别在0.23～1.14μg,0.33～1.64μg范围内线性关系良好,r 值分别为0.9998和0.9999,平均加样回收率分别为97.4%和98.4%。结论:方法操作简便、易行,测定结果稳定、准确,可有效地控制制剂的质量。
Objective: To determine the content of chlorogenic acid and baicalin in Shuanghuanglian injection. Method: HPLC method, column: C 18 column (5μm, 250mm × 4.6mm), mobile phase: methanol - 0.2 mol · L -1 sodium dihydrogen phosphate (PH = pH 2.7) (44 : 56), flow rate: 1.0 mL·min -1, detection wavelength: 320 nm. Results: The chlorogenic acid and baicalin had good linearity in the range of 0.23-1.14 μg and 0.33-1.64-μg, respectively. The r values were 0.9998 and 0.9999, respectively. The average recovery was 97.4% and 98.4%, respectively. Conclusion: The method is simple, easy to operate, stable and accurate, and can effectively control the quality of the preparation.