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A thorough study on the reaction of 32_pN_p with 14 dinucleoside monophosphates including U_pI and C_p m~1I has been reported. The 10μl reaction mixture containing 100mM Tris (pH8.3), 40mMMgCl_2, 6.6mM DIT, bovine serum albumin (20μg/ml), 1mM ATP, 0.25mM dinucleoside monophos-phate, 0.05mM 32_pN_p and T_4 RNA ligase was incubated for 24 hr at 5--10℃ (or 2 hr at 37℃).The joining yields depend on the reaction temperature, the donor species and the base compositionof acceptors,_pC_p is the best donor. At 5--10℃, it can join in all 14 dinucleoside monophosphateswith yields ranging from 20% to 80%. The next one is _pA_p, but the yields are comparatively low.Both _pG_p and p_U_p are poor donors, which react with some dinucleoside monophosphates to give ayield of only a few percent, and sometimes no product could be obtained whatsoever.
A thorough study on the reaction of 32_pN_p with 14 dinucleoside monophosphates including U_pI and C_pm ~ 1 I has been reported. The 10 μl reaction mixture contains 100 mM Tris (pH 8.3), 40 mM MgCl_2, 6.6 mM DIT, bovine serum albumin (20 μg / ml) , 1 mM ATP, 0.25 mM dinucleoside monophos-phate, 0.05 mM 32_pN_p and T_4 RNA ligase was incubated for 24 hr at 5 - 10 ° C (or 2 hr at 37 ° C). The conjugation depend on the reaction temperature, the donor species At 5 - 10 ° C, it can join in all 14 dinucleoside monophosphates with ranging ranging from 20% to 80%. The next one is _pA_p, but the yields are relativelyly low.Both _pG_p and p_U_p are poor donors, which react with some dinucleoside monophosphates to give ayield of only a few percent, and sometimes no product could be achieved whatsoever.