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目的 探讨NO在镉致急性肝损伤中的作用以及慈菇的干预效果。方法 30只雄性SD大鼠随机分为 5组 ,每组 6只 ,分别用 2 5 %、5 0 %、75 %的慈菇 (2ml/ 10 0 g体重 )及等体积蒸馏水灌胃 10天 ,未次灌胃 2 4h后 ,腹腔注射Cd Cl2 (2 0 μmol/L/kg体重 )或蒸馏水 ,染毒 2 4h后 ,观察血清NO含量、GPT、LDH活力及肝组织NOS活力、MDA合量。结果 CdCl2 组与阴性对照组比较 ,血清LDH、GPT活力 ,肝组织MDA合量显著升高 (P <0 0 5 ) ,中、高浓度慈菇干预组相比CdCl2 组LDH、GPT活力及MDA含量显著降低 (P <0 0 5 ) ;血清中NO含量及肝组织NOS活性各组间无明显差异 (P >0 0 5 ) ,不同浓度慈菇对肝组织NOS活性及血清NO浓度的降低具有剂量反应关系 (r分别为 - 0 95 96、-0 995 6 ,P <0 0 5 )。结论 NO可能参与镉所致肝脏的急性过氧化损伤 ,提示慈菇对镉致肝急性损伤具有保护作用
Objective To investigate the role of NO in cadmium-induced acute hepatic injury and the intervention effect of Sagittaria. METHODS: Thirty male Sprague-Dawley rats were randomly divided into 5 groups of 6 rats, which were intragastrically administered with 25%, 50%, 75% Sagittaria (2 ml/100 g body weight) and equal volume of distilled water for 10 days. After intragastric administration for 24 hours, intraperitoneal injection of CdCl2 (20 μmol/L/kg body weight) or distilled water was used for 24 hours. Serum NO, GPT, LDH activity, NOS activity and MDA content in liver tissue were observed. Results Compared with the negative control group, serum LDH, GPT activity and liver tissue MDA content were significantly increased in the CdCl2 group and the negative control group (P < 0.05). LDH, GPT activity and MDA content in the CdCl2 group were higher in medium and high concentrations of Sagittaria Thunb. Significantly decreased (P <0 05); NO content in serum and NOS activity in liver tissue were not significantly different among the groups (P > 0.05). Different concentrations of Sagittaria sinica had a dose-reducing effect on NOS activity and serum NO concentration in liver tissue. Responses (r = - 0 95 96, -0 995 6, P <0 0 5). Conclusion NO may be involved in acute cadmium-induced acute peroxidative injury, suggesting that Sagittaria has a protective effect against cadmium-induced acute liver injury.