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目的 构建日本血吸虫未知基因的原核表达载体 ,研究基因性质。 方法 首先将从日本血吸虫成虫 c DNA文库中筛选得到的未知基因 c DNA JAYL0 2 30亚克隆入原核表达载体 p ET2 8a(+)中 ,IPTG诱导重组蛋白的表达 ,免疫印记实验检测其免疫性质。 结果 成功构建重组原核表达载体 p ET2 8a(+) - JAYL0 2 30 ,并获得稳定表达的融合蛋白 ,免疫印记实验证明该融合蛋白具有免疫反应性。 结论 本实验为进一步深入研究该基因的性质及功能提供了基础
Objective To construct prokaryotic expression vector of unknown gene of Schistosoma japonicum and study its genetics. Methods The unknown gene c DNA JAYL0 2 30 screened from the adult c DNA of Schistosoma japonicum was subcloned into the prokaryotic expression vector p ET2 8a (+). IPTG induced the expression of the recombinant protein. The immunological properties of the recombinant protein were detected by Western blotting. Results The recombinant prokaryotic expression vector p ET2 8a (+) - JAYL0 2 30 was successfully constructed and a stable fusion protein was obtained. Western blotting showed that the fusion protein was immunoreactive. Conclusion This experiment provides the basis for further study on the nature and function of this gene