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目的:探讨人乳头瘤病毒(HPV)各亚型在广西沿海地区宫颈癌患者中的分布情况,HPV感染与宫颈癌患者的年龄、临床分期、病理类型、分化程度、肿瘤盆腔淋巴结转移及肿瘤的复发的关系,为广西沿海地区妇女宫颈癌的防治提供理论依据。方法:通过凯普导流杂交HPV DNA检测法,对76例宫颈癌患者宫颈脱落细胞进行21种HPV亚型的检测。结果:宫颈癌HPV总阳性率为90.8%。宫颈癌患者HPV阳性各亚型出现的频率排序为:HPV16(56.5%),HPV18、33、58各(7.2%),HPV52、53各(5.8%),HPV31(4.3%),HPV45(2.9%),HPV35、51、56、66、68各(1.4%)。HPV6(5.8%),HPV11、44、43各(1.4%)均合并在高危感染中。HPV感染与临床分期、肿瘤分化程度、肿瘤盆腔淋巴结转移及肿瘤的复发差异无显著性(P>0.05),与年龄密切相关,鳞癌HPV阳性率明显高于腺癌及其它癌,差异有统计学意义(P<0.05)。结论:广西沿海地区妇女宫颈癌患者中以HPV16、18、33、58感染为主要型别。HPV感染与宫颈癌的临床分期、肿瘤分化程度、肿瘤盆腔淋巴结转移及肿瘤的复发无明显相关性,与发病年龄、病理类型有关。
Objective: To investigate the distribution of human papilloma virus (HPV) subtypes in patients with cervical cancer in coastal areas of Guangxi. The relationship between HPV infection and cervical cancer patients’ age, clinical stage, pathological type, differentiation degree, pelvic lymph node metastasis and tumor Recurrence of the relationship for the prevention and treatment of cervical cancer in women in coastal areas of Guangxi provide a theoretical basis. METHODS: Twenty-seven HPV subtypes of cervical exfoliated cells from 76 patients with cervical cancer were detected by Kaplan-Meier flow-mediated hybridization with HPV DNA. Results: The total positive rate of HPV in cervical cancer was 90.8%. The frequency of HPV positive subtypes in cervical cancer patients was as follows: HPV16 (56.5%), HPV18,33,58 (7.2%), HPV52,53 (5.8%), HPV31 ), HPV35,51,56,66,68 each (1.4%). HPV6 (5.8%) and HPV11, 44 and 43 (1.4%) were all associated with high-risk infection. HPV infection and clinical stage, tumor differentiation, tumor pelvic lymph node metastasis and recurrence of tumor no significant difference (P> 0.05), and age are closely related to the positive rate of HPV in squamous cell carcinoma was significantly higher than that of adenocarcinoma and other cancers, the difference was statistically Significance (P <0.05). Conclusion: HPV16,18,33,58 women with cervical cancer in coastal areas in Guangxi as the main type of infection. HPV infection and clinical stage of cervical cancer, tumor differentiation, tumor pelvic lymph node metastasis and tumor recurrence no significant correlation with the age of onset, pathological types.