论文部分内容阅读
The translucent endosperm trait in a japonica rice variety‘Kantou 194’is controlled by a Wx-mq gene which is allelic to Wx locus by genetic analysis and allelic test.The amylose content analysis showed that an intermediate amylose content between those of glutinous and non-glutinous rice existed in endosperm of homozygous Wx-mq genotype.The slight changes of amylose content in different varieties and F1 grains with an identical Wx-mq genotype might be influenced by dissimilar genetic background.To identify the Wx-mq genotype simply and rapidly,a cleaved amplified polymorphic sequence(CAPS) marker was designed.The result from the molecular detection indicated that it could be used for marker-assisted selection for low amylose content varieties in rice breeding.
The translucent endosperm trait in a japonica rice variety ’Kantou 194’is controlled by a Wx-mq gene which is allelic to Wx locus by genetic analysis and allelic test. Amylose content analysis showed that an intermediate amylose content between those of glutinous and non -glutinous rice existed in endosperm of homozygous Wx-mq genotype. slight changes of amylose content in different varieties and F1 grains with the same Wx-mq genotype might be influenced by dissimilar genetic background. To identify the Wx-mq genotype simply and rapidly , a cleaved amplified polymorphic sequence (CAPS) marker was designed. The result from the molecular detection indicated that it could be used for marker-assisted selection for low amylose content varieties in rice breeding.