论文部分内容阅读
目的:利用蛋白质组学技术筛选重度子痫前期患者尿液中潜在的特异性生物标志物。方法:利用荧光差异蛋白表达(2-D DIGE)与MALDI-TOF质谱联用的方法筛选并鉴定重度子痫前期患者和正常妊娠妇女尿液中的差异蛋白质,并验证两组孕妇尿液中的蛋白质差异。结果:和正常妊娠组相比较,重度子痫前期组总蛋白水平显著增高;重度子痫前期组和正常妊娠组有显著表达差异的蛋白点65个,纤维蛋白原α链和胶原α链在两组中有显著差异。结论:纤维蛋白原α链和胶原α链可能参与了子痫前期的发病过程,作为重度子痫前期患者的早期诊断标准具有良好的前景。
OBJECTIVE: To screen potential specific biomarkers in urine of patients with severe preeclampsia using proteomic techniques. Methods: The differential proteins in urine of patients with severe preeclampsia and normal pregnant women were screened and identified by 2-D DIGE and MALDI-TOF mass spectrometry. Protein differences. Results: Compared with normal pregnancy group, the total protein level in severe preeclampsia group was significantly increased. There were 65 protein spots with significant difference between severe preeclampsia group and normal pregnancy group. The fibrinogen α chain and collagen α chain were in two There were significant differences among the groups. CONCLUSION: The α-chain of fibrinogen and α-chain of collagen may be involved in the pathogenesis of preeclampsia and have a good prospect as an early diagnostic criterion in patients with severe preeclampsia.