论文部分内容阅读
研究ｒｔ－ＰＡ工程细胞在小型填充床反应器中连续高密度培养的过程，以及在连续灌注培养条件下ｒｔ－ＰＡ表达水平、葡萄糖的消耗率、乳酸和氨与ｒｔ－ＰＡ表达水平之间的关系以探索该工程细胞培养的最佳条件。在不中断培养的情况取样作细胞计数、ｒｔ－ＰＡ和代谢物的检测。结果：ｒｔ－ＰＡ工程细胞在小型填充床反应器中最高细胞数可达３．８×１０９细胞，最高ｒｔ－ＰＡ浓度达１２ｍｇ／Ｌ结论：这种新型的小型填充床反应器是操作方便、利用价值高的研究设备。
The process of continuous high-density culture of rt-PA engineered cells in a small packed bed reactor was studied as well as the level of rt-PA expression, glucose consumption, lactate and ammonia and rt-PA expression levels under continuous perfusion culture Relationship to explore the best conditions for cell engineering in this project. Samples were taken for cell count, rt-PA and metabolite detection without interruption of culture. RESULTS: rt-PA engineered cells reached a maximum of 3.8 × 109 cells in a small packed bed reactor with a maximum rt-PA concentration of 12 mg / L Conclusion: This new small packed bed reactor is easy to operate, Use high-value research equipment.