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本文将凹度、规化形状因子和线参照数密度新参数用于大肠癌定量病理学研究。病例分为正常大肠粘膜、大肠腺瘤和大肠管状腺癌三组。用Q500 MC 显微图像分析系统测试腺体的面积、周长、长轴、短轴, 并分别计数各腺体周长上的腺上皮细胞数和杯状细胞数。据此计算腺体的体凹度、表凹度、面凹度、边凹度、规化形状因子和腺上皮细胞的线参照数密度及杯状细胞的线参照数密度。结果表明, 腺体的体凹度、表凹度、面凹度、边凹度、规化形状因子和以基底膜为参照系的腺上皮细胞的线参照数密度和杯状细胞的线参照数密度在大肠正常粘膜、腺瘤和管状腺癌之间的差异有显著性( P< 0-05) 。基于本文测试结果, 我们发现癌性腺体和腺瘤性腺体在定量病理学方面具有以下特点, 即: 癌性腺体其腺上皮细胞的线参照数密度明显下降, 绝大部分病例中腺体的杯状细胞消失, 其线参照数密度为0 或接近0 , 此外腺体的凹度增加, 规化形状因子值下降; 腺瘤性腺体其腺上皮细胞的线参照数密度明显增加, 腺体凹度的增加程度和RFF值下降的程度较癌性腺体小。本研究表明体凹度、表凹度、面凹度、边凹度、规化形状因子和曲线上的线参照数密度在病理学定理分析中有实际应用价值
In this paper, new parameters of densities, shape factors, and line reference densities were used for quantitative pathological studies of colorectal cancer. Cases were divided into three groups: normal colon mucosa, colorectal adenoma, and large intestine tubular adenocarcinoma. The area, perimeter, major axis, and minor axis of the glands were measured with a Q500 MC microscopic image analysis system, and the number of glandular epithelial cells and the number of goblet cells on the perimeter of each gland were counted, respectively. Based on this, the volume of concavity, surface concavity, facial concavity, side concavity, morphological shape factor, and linear reference number density of glandular epithelial cells and line reference number density of goblet cells were calculated. The results showed that the density of glands, the degree of surface concavity, the degree of surface concavity, the degree of lateral concavity, the morphological shape factor, and the linear reference number density of glandular epithelial cells based on the basement membrane and the number of line reference of goblet cells The density was significantly different between normal mucosa, adenoma and tubular adenocarcinoma (P < 0-05). Based on the test results of this paper, we found that cancerous glands and adenomatous glands have the following characteristics in quantitative pathology, namely: Cancer glands have a marked decrease in the linear reference number density of glandular epithelial cells, and most of the cases are glandular. The goblet cells of the body disappeared and the linear reference number density was 0 or close to 0. In addition, the concavity of the gland increased, and the value of the morphological shape factor decreased; the density of the line reference number of glandular epithelial cells in the adenoma glands increased significantly. The extent of glandular concavity increase and the extent of RFF decrease are smaller than those of cancerous glands. This study shows that the body concavity, surface concavity, surface concavity, edge concavity, shape factor, and line reference density on the curve have practical application value in pathological theorem analysis.