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目的研究豚鼠耳蜗组织中缝隙连接结构以及连接蛋白26和32分布及其意义。方法分别采用透射电镜和免疫组织化学方法观察豚鼠耳蜗中缝隙连接及连接蛋白26和32的分布情况。结果在豚鼠的内耳中,尤其是在支持细胞和毛细胞间存在着大量的缝隙连接结构。连接蛋白26广泛分布在毛细胞和支持细胞间、螺旋缘和血管纹区域内;连接蛋白32则在毛细胞下支持细胞间质中,以及血管纹区域,而螺旋神经结处无分布。结论豚鼠内耳中也存在着大量的缝隙连接结构,两种连接蛋白在其内耳中均有分布,其中连接蛋白26是主要种类。
Objective To study the distribution of gap junction and the distribution of connexin 26 and 32 in cochlear of guinea pig and its significance. Methods Transmission electron microscopy and immunohistochemical methods were used to observe the distribution of gap junction and connexin 26 and 32 in cochlea of guinea pigs. As a result, there are a large number of gap junction structures in the inner ear of guinea pig, especially between the supporting cells and the hair cells. Connexin 26 is widely distributed in hair cells and supporting cells, helical edges and stria vascularis; connexin 32 supports interstitial and striated regions in the stroma under the hair cells, whereas there is no distribution at the spiral nerve junction. Conclusion There are also a large number of gap junctional structures in the inner ear of guinea pigs. Both connective proteins are distributed in the inner ear. Connexin 26 is the main type.