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从用药水平较低地区棉田中采集棉蚜,在室内液培棉苗上繁殖,分别用氰戊菊酯、氧乐果及氰戊菊酯+氧乐果(1:8?AI)混剂以浸蘸法处理试虫16代,氰戊菊酯选育的种群抗性高达4 324.4倍,而氧乐果及混剂选育的抗性仅分别为223倍及16.5倍。上述三种抗性种群对溴氰菊酯、氯氟氰菊酯和氰戊菊酯均分别表现出显著或高度交互抗性,但对一些有机磷、氨基甲酸酯及有机氯杀虫剂的交互抗性却不明显。用酶抑制剂生测法及离体酶活性测定的研究表明,棉蚜对上述药剂的抗性与羧酸酯酶及氧化酶活力增加有关。
Aphis gossypii was collected from cotton fields with lower drug levels and then propagated on indoor cotton seedlings. Fenvalerate, omethoate and fenvalerate and omethoate (1: 8? AI) The dipping method was used to deal with 16 generations of test insects, fenvalerate breeding population resistance as high as 4 324.4 times, and omethoate and mixture breeding resistance were only 223 times and 16.5 times. The above three resistant populations showed significant or highly interactive resistance to deltamethrin, cyhalothrin and fenvalerate, respectively, but for some organophosphorus, carbamate and organochlorine pesticides Interactive resistance is not obvious. The results of enzyme assays and in vitro enzymatic activity assays showed that the resistance of cotton aphids to these agents was related to the increase of carboxylesterase and oxidase activities.