论文部分内容阅读
磷酸盐(PO_4)的比色测定,现有钼蓝法和钒黄法两种。血液中无机磷的比色测定,在临床化学检验上向来采用钼蓝法。但是根据文献的记载,钼蓝法的显色常受到某些条件的限制,如酸度、还原剂用量等均有影响,颜色的稳定性也不够理想.钒黄法是基于磷酸根在钒酸铵和钼酸铵的酸性溶液中生成黄色格合物,它属于磷钼钒酸类H_7[P(Mo_2O_7)_n
The colorimetric determination of phosphate (PO_4) has two methods: molybdenum blue and vanadium yellow. The colorimetric determination of inorganic phosphorus in blood has traditionally been molybdenum blue. However, according to the literature, the coloration of the molybdenum blue method is often limited by certain conditions, such as acidity, reducing agent dosage, etc., and the color stability is not ideal. The vanadium yellow method is based on phosphate in the ammonium vanadate In the acidic solution of ammonium molybdate, a yellow complex is formed, which belongs to the group of molybdovanovanic acids H_7[P(Mo_2O_7)_n