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目的探讨caspase募集结构域家族成员18(CARD18)在凋亡素诱导胃癌细胞凋亡的机制及在胃癌发生发展中的作用。方法运用二维凝胶电泳法分离凋亡素作用胃癌细胞后表达的差异蛋白质,通过基质辅助激光解吸附飞行时间质谱技术(MALDI-TOF-MS)和生物信息学方法确定其中部分差异蛋白质,RT-PCR和Western blot法检测CARD18的表达,采用免疫组化检测56例胃癌组织及癌旁组织中CARD18的表达,并分析其与胃癌临床病理参数的关系。结果凋亡素处理后的胃癌细胞CARD18表达下调最明显,CARD18蛋白在胃癌组织中的表达明显高于癌旁组织(P<0.05),且与胃癌的分化程度和淋巴结转移有关(P<0.05)。结论 CARD18可作为一个潜在的预后标志物和靶向标志物。
Objective To investigate the mechanism of apoptosis induced by apoptin in gastric cancer cells and its role in the development of gastric cancer. Methods Two-dimensional gel electrophoresis (SDS-PAGE) was used to separate the differentially expressed proteins after apoptosis of gastric cancer cells. Some differential proteins were identified by using MALDI-TOF-MS and bioinformatics methods. RT The expression of CARD18 was detected by PCR and Western blot. The expression of CARD18 was detected by immunohistochemistry in 56 gastric cancer tissues and adjacent tissues. The relationship between the expression of CARD18 and clinicopathological parameters was also analyzed. Results The expression of CARD18 was significantly down-regulated in apoptin-treated gastric cancer cells. The expression of CARD18 protein in gastric carcinoma was significantly higher than that in paracancerous tissues (P <0.05), and correlated with the differentiation and lymph node metastasis (P <0.05) . Conclusion CARD18 can be used as a potential prognostic marker and target marker.