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目的:探讨皂角提取物对微循环障碍和血栓形成的作用。方法:采用电刺激法制备大鼠颈总动脉栓塞模型,按Rayer法制备大鼠下腔静脉栓塞模型。大鼠手术前连续给药3天(皂角提取物150mg/kg、75mg/kg、38mg m l/kg),测定电刺激致大鼠颈总动脉血栓形成所需时间及静脉血栓重量。采用肾上腺素加冰水浴法复制大鼠急性微循环障碍模型,造模后24h(第5日)用激光多普勒测定耳廓灌注量(PU)、运动的血细胞浓度(CMBC)及血细胞的运动速率(V),同时进行尾静脉取血,测定给药后红细胞计数(RBC)、红细胞平均体积(MCV)及红细胞体积分布宽度(RDW-CV),血小板计数(PLT)及血小板平均体积(MPV)、血小板体积分布宽度(PDW)。结果:与模型组比,皂角提取物给药后血栓湿重、干重明显降低,皂角提取物各剂量组给药后血栓形成时间有不同程度延长。与模型组比,皂角提取物各剂量组耳廓灌注量(PU)、运动血细胞浓度(CMBC)、血细胞运动速率(V)均明显增加。与模型组比,皂角提取物各剂量组均显著降低红细胞平均体积(MCV)及体积分布宽度(RDW-CV);但对血小板计数(PLT)无明显影响,皂角提取物各剂量组血小板平均体积(MPV)及血小板体积分布宽度(PDW)均明显降低。结论:皂角提取物能抑制血栓形成和改善微循环障碍。
Objective: To investigate the effect of saponin extract on microcirculatory disturbance and thrombosis. METHODS: Rat models of common carotid artery embolization were prepared by electrical stimulation. Rat inferior vena cava thrombosis model was established by Rayer method. The rats were continuously given 3 days before surgery (sweetness extract 150 mg/kg, 75 mg/kg, 38 mg ml/kg), and the time required for the thrombosis of the common carotid artery induced by electrical stimulation and the weight of the venous thrombus were measured. A rat model of acute microcirculatory disturbance was replicated using an adrenaline and ice-water bath method. Auricle perfusion (PU), exercise blood cell concentration (CMBC) and exercise of blood cells were measured by laser Doppler 24 hours after the model was established (Day 5). Velocity (V), simultaneous tail vein sampling, measurement of red blood cell count (RBC), mean red blood cell volume (MCV), and erythrocyte volume distribution width (RDW-CV), platelet count (PLT) and mean platelet volume (MPV) after administration ) Platelet volume distribution width (PDW). RESULTS: Compared with the model group, the wet weight and dry weight of the thrombus were significantly reduced after the administration of the saponin extract, and the thrombus formation time of each dose group of the saponin extract was prolonged. Compared with the model group, auricle perfusion (PU), exercise blood cell concentration (CMBC), and blood cell movement rate (V) were significantly increased in each dose group of the saponin extract. Compared with the model group, the average volume of red blood cells (MCV) and width of distribution (RDW-CV) were significantly reduced in each dose group of Glycyrrhizae sinensis extract; but there was no significant effect on platelet count (PLT). The mean volume (MPV) and platelet volume distribution width (PDW) were significantly reduced. Conclusion: Saponin extract can inhibit thrombosis and improve microcirculation.