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在进行研究支气管炎工作中,为了观察组织病变深度和广度的改变,连续切片观察是具有一定意义的,由于制作过程中切片量多,染色时亦有一定困难。因此,考虑到可试作组织块直接染色法,可避免脱落现象,故试作此法,将切片贴于载片上脱腊后即盖上盖片进行观察。1.将已经完成固定的肺组织切取厚2-3毫米,充分水洗。2.倾入二十倍于组织体积的苏木素(Harri’s)液内浸染2-3天,每天轻压一次
In the study of bronchitis, in order to observe changes in the depth and breadth of tissue lesions, continuous slicing observation is of some significance. Due to the large number of slices in the production process, there are certain difficulties in dyeing. Therefore, taking into account that you can try to direct tissue staining method, to avoid the phenomenon of shedding, so try this method, will be attached to the slide on the slide after dewaxing covered with a cover sheet for observation. 1. Take the fixed lung tissue and cut 2-3 mm thick. Wash thoroughly. 2. Pour into Harri’s solution twenty times the volume of the tissue for 2-3 days, gently press once a day