Fine Mapping and Cloning of Leafy Head Mutant Gene pla1-5 in Rice

• 作 者: FENG Gong-neng ZHANG Chang-quan ZHAO Dong-sheng ZHU Kong-zhi TU Huai-zhou XU Chen-wu LIU Qiao-quan
• 机 构: Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture, Ya
• 出 处: Rice Science
• 发表日期: 2013年05期
• 关键词: mutant phenotype recessive chromosome cultivar deletion panicle Mutant encoding

We identified a leafy head mutant pla1-5(plastochron 1-5)from the progeny of japonica rice cultivar Taipei 309 treated with60Co-γray irradiation.The pla1-5 mutant has a dwarf phenotype and small leaves.Compared with its wild type,pla1-5 has more leaves and fewer tillers,and it fails to produce normal panicles at the maturity stage.Genetic analysis showed that the pla1-5 phenotype is controlled by a single recessive nuclear gene.Using the map-based cloning strategy,we narrowed down the location of the target gene to a 58-kb region between simple sequence repeat markers CHR1027 and CHR1030on the long arm of chromosome 10.The target gene cosegregated with molecular markers CHR1028 and CHR1029.There were five predicted genes in the mapped region.The results from sequencing analysis revealed that there was one base deletion in the first exon of LOC_Os10g26340 encoding cytochrome P450 CYP78A11 in the pla1-5 mutant,which might result in a downstream frame shift and premature termination.These results suggest that the P450 CYP78A11 gene is the candidate gene of PLA1-5.

We identified a leafy head mutant pla1-5 (plastochron 1-5) from the progeny of japonica rice cultivar Taipei 309 treated with 60Co-γray irradiation.The pla1-5 mutant had a dwarf phenotype and small leaves. Compared with its wild type, pla1 -5 has more leaves and fewer tillers, and it fails to produce normal panicles at the maturity stage. Genetic analysis showed that the pla1-5 phenotype is controlled by a single recessive nuclear gene. Using the map-based cloning strategy, we narrowed down the location of the target gene to a 58-kb region between simple sequence repeat markers CHR1027 and CHR1030 on the long arm of chromosome 10. The target gene cosegregated with molecular markers CHR1028 and CHR1029. There were five predicted genes in the mapped region. The results from sequencing analysis revealed that there was one base deletion in the first exon of LOC_Os10g26340 encoding cytochrome P450 CYP78A11 in the pla1-5 mutant, which might result in a downstream frame shift and premature termination. e results suggest that the P450 CYP78A11 gene is the candidate gene of PLA1-5.