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采用ＳＤ大鼠２０只，１５只为实验组，５只作为对照组。用微量注射器将６－羟基多巴８μｇ／μｌ分别注入大鼠右侧黑质致密部和中脑腹侧背盖区，观察大鼠行为变化及黑质和纹状体形态学改变。结果：注药４周后，实验组动物经阿朴吗啡诱发１４只出现旋转，超过７转／分为１２只（占８０％），经反复测试结果稳定；旋转鼠行ＮｉｓⅠ染色发现右侧黑质致密部和中脑腹侧背盖区神经元数目明显减少；酪氨酸羟化酶免疫组织化学染色见右侧黑质致密部及纹状体区ＴＨ免疫反应数目较对侧明显下降。对照组则无旋转行为和形态学改变。提示：采用改进的方法建立大鼠帕金森氏病模型可明显提高成功率。
Twenty Sprague-Dawley rats were used, 15 rats in the experimental group and 5 rats in the control group. The 6-hydroxydopamine 8μg / μl was injected into the substantia nigra pars compacta and the ventral tegmental area of the right ventral part of the rat with a microinjector to observe the behavioral changes and the morphological changes of the substantia nigra and striatum. Results: Four weeks after injection, 14 animals induced by apomorphine appeared more than 7 rotations / divided into 12 animals (80%), which were stable after repeated tests. NisI staining showed that the right black The number of neurons in the substantia nigra pars compacta and mesencephalic ventral tegmental area was significantly decreased. The number of TH immunoreactions in the substantia nigra pars compacta and striatum on the right side was significantly lower than that on the contralateral side by tyrosine hydroxylase immunohistochemical staining. The control group had no rotational behavior and morphological changes. Tip: Using an improved method to establish Parkinson’s disease rat model can significantly improve the success rate.